Droplet size and deposition of aerosolized AAV in a mouse snout-only study

ISAM 2025 -- Clinical approval of an inhaled therapy often requires preclinical evaluation using a small and large animal model. Small animal dosing is undertaken using a snout-only inhalation chamber and specifically for adeno-associated viruses (AAVs) is contained within an ABSL2 designated area. Male C57BL/6J mice were administered AAV6.2-mCherry aerosol at achieved aerosol concentrations determined by quantitative polymerase chain reaction (qPCR). Swab samples from various locations external to the inhalation exposure system were collected and analyzed using qPCR to test for laboratory contamination. Samples collected gave negligible values of AAV. The data presented demonstrates the feasibility of aerosolizing AAV6.2-mCherry for inhalation exposure and that ABSL 2 procedures are required to ensure cleanliness and minimize the risk of cross-contamination.