Variability in vibrating mesh nebulizer aerosol concentration during nonclinical use

ISAM 2025 -- Clinical nebulizer output is commonly directed into a mouthpiece or patient facemask, whereas nonclinical nebulizer output is directed into a stream of carrier air going to the animal breathing zone. Nonclinical inhalation toxicology studies involve delivering an aerosol to multiple animals simultaneously for up to 6 hours/day, so the aerosol generation device must produce a stable output. The vibrating mesh nebulizer is an appropriate device for formulations with fragile active ingredients such as antibodies and lipid nanoparticles. Aerosol samples are collected from the breathing zone of the animals. Analysis allows for determination of the delivered dose, which is important for calculating clinical safety margins.